Scale of synthesis |
0.02 µmol |
0.05 µmol |
0.20 µmol |
1.0 µmol |
15 µmol |
Length restriction |
N/A |
46-80 |
10-80 |
10-80 |
n/a |
Minimum yield 20 nucleotides |
N/A |
5 OD |
12 OD |
60 OD |
|
Desalting removes residual by-products from the synthesis, cleavage, and deprotection procedures. For many applications (including PCR), desalting is fine for oligos <= 30 bases; the overwhelming abundance of full length oligo outweighs any contributions from shorter oligos. For all oligos >30 bases in length, it is recommended that an additional method of purification be considered.
 |
Fig. 1 RP-HPLC chromatogram of a crude M13Rev primer purified by desalting. Notice two large peaks at 3 and 5 minutes. These are contaminations due to failure products. These contaminants are almost completely eliminated when purified with RP-cartridge purification. |
