CyberGene provides High Quality Dual-Labelled Probes for quantitative PCR (qPCR). Each oligo can be supplied in any synthesis scale. Reverse-phase HPLC is recommended.
Dual-Labelled Probes are delivered either lyophilized or in aqueous solution and concentration is measured by spectrophotometer. The Oligos are shipped in 2 ml screw cap tubes within 4-7 working days.
Quenchers
| |
Quenching range nm |
Comment |
BHQ-1* |
480-580 |
Dark quencher |
BHQ-2* |
550-650 |
Dark quencher |
| TAMRA |
500-580 |
|
| Dabcyl |
400-530 |
Dark quencher |
| DDQ-1 |
400-530 |
|
BBQ-650 |
550-750 |
|
Available reporter dye/quencher combinations
Reporter dyes 5' |
BHQ-1 |
BHQ-2 |
BBQ-650 |
TAMRA |
Dabcyl |
Emission max. nm* |
Excitation max.nm* |
6-FAM |
x |
- |
- |
x |
x |
520 |
495 |
HEX |
x |
- |
- |
x |
x |
556 |
535 |
TET |
x |
- |
- |
x |
x |
536 |
521 |
Y.Yellow** |
x |
- |
x |
- |
x |
548 |
525 |
Cy3 |
- |
x |
x |
- |
- |
570 |
550 |
Cy5 |
- |
x |
x |
- |
- |
670 |
649 |
TAMRA |
- |
x |
x |
- |
- |
576 |
555 |
TexasRed |
- |
x |
x |
- |
- |
603 |
583 |
| Rox |
- |
x |
x |
- |
- |
574 |
602 |
| Atto 550 |
- |
x |
x |
- |
- |
579 |
550 |
| Atto 565 |
- |
x |
- |
- |
- |
592 |
563 |
| Atto 633 |
- |
x |
- |
- |
- |
657 |
629 |
* These are aproximately values and they may change according to actual experimental conditions
** Replacement for VIC (ABI)
If you miss a reporter dye please let us know. Send an e-mail to oligo-syntes@cybergene.se
Trademarks
Texas Red is a trademark of Molecular Probes, Inc. for R&D use only
Black Hole Quenchers (BHQ) are registered trademarks of Biosearch Techologies, Novato, CA, USA – for R&D use only
Cy3 and C5 are registered trademarks of Amersham Biosciences Corp., includes a limited non-exclusive sublicence under US Patent No.s 5 556 959, 5 808 044. Commercial use is strictly forbidden without written permission from Amersham Biosciences
Yakima Yellow is a registered trademark of Epoch Biosciences Inc. For R&D only, not for commercial, clinical, diagnostic or any other use. For commercial use a license must be obtained from Epoch Biosciences.
